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Table 3 Summary of studies using formalin-fixed human renal biopsies for glomerular micro-dissection and gene expression analysis of glomerular mRNA

From: Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples

First author

Sections cut in (µm)

Staining method

Number of micro-dissected glomerular transections

RNA extraction

RNA purity (A260/A280 ratio)

Total RNA quantity used for cDNA synthesis

Preamplification

Glomerular reference gene(s)

Lit.

Cohen

5

Hemalaun

20–50

Phenol–chloroform

nd

nd

None

GAPDH

[10]

Fries

5

None

10–25

Purescript RNA isolation kit (Gentra, Minneapolis, MN)

nd

nd

None

ACTB

[12]

Schmid

5

Hemalaun

20–50

RNeasy-Mini

nd

nd

None

GAPDH and 18 S rRNA

[11]

Landolt

10

HE

100

Various were compared

1.1–1.9

54–138 ng

Only pre-analytical steps were performed

[9]

Bao

Not given

None

50

mirVana miRNA Isolation Kit

nd

nd

None

ACTB

[13]

Bockmeyer

2

Comparing no staining and Hemalaun

Comparing 20–300

Phenol–chloroform

0.9 to 2.3

157–282 ng

Yes

PGK1, PPIA

This study

  1. nd not determined