First author | Sections cut in (µm) | Staining method | Number of micro-dissected glomerular transections | RNA extraction | RNA purity (A260/A280 ratio) | Total RNA quantity used for cDNA synthesis | Preamplification | Glomerular reference gene(s) | Lit. |
---|---|---|---|---|---|---|---|---|---|
Cohen | 5 | Hemalaun | 20–50 | Phenol–chloroform | nd | nd | None | GAPDH | [10] |
Fries | 5 | None | 10–25 | Purescript RNA isolation kit (Gentra, Minneapolis, MN) | nd | nd | None | ACTB | [12] |
Schmid | 5 | Hemalaun | 20–50 | RNeasy-Mini | nd | nd | None | GAPDH and 18 S rRNA | [11] |
Landolt | 10 | HE | 100 | Various were compared | 1.1–1.9 | 54–138 ng | Only pre-analytical steps were performed | [9] | |
Bao | Not given | None | 50 | mirVana miRNA Isolation Kit | nd | nd | None | ACTB | [13] |
Bockmeyer | 2 | Comparing no staining and Hemalaun | Comparing 20–300 | Phenol–chloroform | 0.9 to 2.3 | 157–282 ng | Yes | PGK1, PPIA | This study |