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Table 3 CRISPR Cas9 mutation frequencies in mammalian cell lines

From: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone

Cell line

G418-resistant isolates screened by western blot analysis

Number of homozygous truncation mutants

Homozygous mutation frequency (%)

U87MG Trial 1

77

4a

5

U87MG Trial 2

50

3a

6

BT549

56

5

9

HEK293

77

1

1.3

  1. aThese mutants are shown in western blot analyses found in Figs. 4, 5