From: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone
Cell line
G418-resistant isolates screened by western blot analysis
Number of homozygous truncation mutants
Homozygous mutation frequency (%)
U87MG Trial 1
77
4a
5
U87MG Trial 2
50
3a
6
BT549
56
9
HEK293
1
1.3