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Table 4 Primers utilized to detect and sequence FOXO3 gene disruption

From: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone

Primer name

Sequence

FOXO3 F (for detection of disruption)

5′-GTGCTTCAGGATCGCTTCA-3′

Neo cassette R (for detection of disruption)

5′-TGCATGCTTTGCATACTTCTG-3′

FOXO3 seq. (for sequencing disruption mutants)

5′-CTCGGTTTTGGACCATTCTG-3′

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BMC Molecular Biology

ISSN: 1471-2199

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