Fig. 5
From: Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides
Silencing effect of the AMOs in MKN74 cells. Relative expression levels (measured by qRT-PCR) of a miR-9 and b CDH1 mRNA, after 4, 8, 12 and 24 h treatment with AM_LNA_PS, AM_LNA_2OMe_PS, and SC_LNA_PS. c Western blot analysis of the relative variation of E-cadherin expression in MKN74 cells after treatment with LNA-anti-miR oligonucleotides and the control, MKN74 cells without any treatment, at the same time-points used for qRT-PCR. All the qRT-PCR and western blot results were normalized against the MKN74 control and the levels of the respective endogenous control (RNU48 for miR-9 and GAPDH for CDH1 gene quantified by qRT-PCR and E-cadherin protein expression by western blot)