Fig. 5

a–c PC4 protein level and its interaction with CstF64 is constant during the cell cycle. a Western blot followed by immunodetection with anti-actin and anti-PC4 antibodies were performed using protein extracts isolated from asynchronous HeLa cells (AS) and cells synchronized to G1 and S phase. b Phos-tag™-based protein electrophoresis followed by Western blot and immunodetection with anti-PC4 antibodies using protein extracts isolated from asynchronous HeLa cells (AS) and cells synchronized to G1 and S phase. c Protein extracts isolated from cells synchronized to G1 and S phase were subjected to immunoprecipitation with anti-PC4 antibodies conjugated to protein A-magnetic beads or non-conjugated protein A-magnetic beads (mock) followed by Western blot and immunodetection with antibodies as indicated on the right. d–f PC4 influences cell proliferation. Charts represent the mean number of cells with PC4 KD (d) and PC4 OE (e) (n = 3). Additional cell proliferation test using MTT assay was performed on PC4 KD cells (f). HeLa scramble and PC4 KD cells were cultured with addition of doxycycline, 1st day of experiment represents 1st day of culture with doxycycline from the day the experiment started