Fig. 3From: The Dictyostelium discoideum homologue of Twinkle, Twm1, is a mitochondrial DNA helicase, an active primase and promotes mitochondrial DNA replicationRelative twm1 mRNA levels of D. discoideum AX2 in response to EtBr treatment. Initial AX2 cultures (T0) were first treated with 10 μg/ml EtBr for 24 h (T24), at which point the EtBr-containing medium was removed and replaced. Following this, cells were allowed a further 24 h to recover without EtBr (T48). A duplicate culture without EtBr treatment was used as a control. twm1 mRNA levels were quantified with qRT-PCR and normalized against a structural gene (tubB). Values are relative to initial (T0) mRNA level. Error bars represent the standard error. p values were calculated using Student’s t-test (*< 0.05; **< 0.0001)Back to article page