Fig. 7
The positions of H2O2-induced chromosome breaks within the AF9 SAR region. a The AF9 genomic map from nucleotide positions 601–281,480 [EMBL:ENSG00000171843] [26]. Black vertical lines represent the locations of exons 1–10. Green boxes show the two previously identified patient BCRs, namely BCR1 and BCR2 [28]. Yellow boxes show the two MAR/SARs which were extracted experimentally in the previous study. These two MAR/SARs were indicated as SAR1 and SAR2 [28]. BamH I (B) restriction sites are shown. Yellow arrows represent the potential MAR/SAR sites predicted by MRS in our study. b The AF9 SAR region (10.2 kb). BamH I (B), Kpn I (K) and Nde I (N) restriction sites are shown. Green and blue arrows represent the primers used in the first and second rounds of nested IPCR, respectively. c Breakpoints mapped in H2O2-treated cells. Red and green vertical lines show the breakpoints identified in H2O2-treated NP69 and HK1 cells, respectively. All the chromosome breaks were mapped within BCR1 which is bordered SAR1 and SAR2. Blue box represents the AF9 region (at coordinates 245,252–245,612) that was previously reported to translocate with the MLL gene resulting in the formation of the MLL-AF9 fusion gene in an ALL patient [GenBank:AM050804]