Fig. 4From: Molecular analysis of NPAS3 functional domains and variantsNPAS3 and ARNT regulate expression of TXNIP and VGF. a Relative quantity of mRNA as assessed by qRT-PCR in HEK 293T cells expressing NPAS3 or ARNT. Expression was assessed 48 h post-transfection, with media replacement 24 h post-transfection and without. VGF and TXNIP were found to be regulated in response to expression of NPAS3 and ARNT. *p < 0.05, **p < 0.01, ***p < 0.001. Data are representative of three biological replicates. b Western blot demonstrating expression of constructs indicatedBack to article page