Fig. 5From: HexA is required for growth, aflatoxin biosynthesis and virulence in Aspergillus flavusDeletion of hexA results in reduced AF production. a AFB1 production ofWT, ΔhexA and ΔhexAC were detected by TLC after cultured in YES liquid media for 6 days. b Quantification of AFB1 production as in a. c qPCR result of AF biosynthetic related genes (aflK, aflD, aflR and aflS) at 72 h. Each bar indicates the mean ± standard deviation (SD) of four replicate assay experiments. ***Significant difference between the WT and mutant strains at P < 0.001, as assessed by one-way ANOVA and Dunnett’s multiple-comparisons testBack to article page