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Table 2 Primers used for the strain construction in this study

From: HexA is required for growth, aflatoxin biosynthesis and virulence in Aspergillus flavus

Primers

Sequence (5′–3′)

Description

hexA-A-F

TCCTCGTAACTTTATCACCG

hexA deletion

hexA-A-R

GGGTGAAGAGCATTGTTTGAGGCTTCGTTCTTCACGGGTTG

hexA-B-F

GCATCAGTGCCTCCTCTCAGACCTCAACGCCTCGTAAACA

hexA-B-R

GTGGCAGCAAGAGTATGG

hexA-ORF-F

TCCATTCAGCCAGCAACA

hexA-ORF-R

ACACGGGCGTCAAAGTCC

PyrG/F

GCCTCAAACAATGCTCTTCACCC

HexA deletion (A. fumigatus pyrG)

PyrG/R

GTCTGAGAGGAGGCACTGATGC

P801-R

CAGGAGTTCTCGGGTTGTCG

hexA mutant screen

P1020-F

ATCGGCAATACCGTCCAGAAGC

hexA-O-F

TGGGTTATGCCTGTTCTGG

hexA mutant screen

hexA-O-R

GGTTTCTTTCTCGGGTGC

Step1-F

GGGCAGTAGGTATTGTAGGT

hexA complementation

Step1-R

CCTCGGTTCACTACAGCAC

ORF-UTR-F

TCAAGAGCCTACCTCATACC

ORF-UTR-R

GGGTGAAGAGCATTGTTTGAGGCACAAGCCACTTCCACCC

UTR-F

GCATCAGTGCCTCCTCTCAGACTCAACGCCTCGTAAACA

UTR-R

TGGCAGCAAGAGTATGG

Overlap-F

AGCCAGTCAACCCACCT

Overlap-R

CTTACGGCATTAGTACATTTCT