Table 3 Primers used for qPCR
From: HexA is required for growth, aflatoxin biosynthesis and virulence in Aspergillus flavus
Primers | Sequence (5′–3′) | Description |
|---|---|---|
hexA/QF | CTCTTCACCCGCCAACT | hexA qPCR |
hexA/QR | CGCCCTGAGGAACAACT | |
aflK/QF | GAGCGACAGGAGTAACCGTAAG | aflK qPCR |
aflK/QR | CCGATTCCAGACACCATTAGCA | |
aflD/QF | TGTATGCTCCCGTCCTACTGTTTC | aflD qPCR |
aflD/QR | TGTAGTCTCCTTAGTCGCTTCATC | |
aflS/QF | CGAGTCGCTCAGGCGCTCAA | aflS qPCR |
aflS/QR | GCTCAGACTGACCGCCGCTC | |
aflR/QF | AAAGCACCCTGTCTTCCCTAAC | aflR qPCR |
aflR/QR | GAAGAGGTGGGTCAGTGTTTGTAG | |
actin/QF | ACGGTGTCGTCACAAACTG | actin qPCR |
actin/QR | CGGTTGGACTTAGGGTTGATAG |