Table 4 Details of primers used for amplification of RNAi pathway genes
Gene name | Sequence information | Primer Name | Primer Sequence (5′→3′) |
|---|---|---|---|
SID-1 | CDS_23342_transcript_23605 | qTt_SID1_F | TGTTGCTTGGTGCGATACT |
qTt_SID1_R | GGTCAGAGCTGTGTTGAGATAA | ||
Dicer-1 | CDS_24712_transcript_26352 | qTt_Dcr1_F | TCTGCTTCTCGCTACGTTATG |
qTt_Dcr1_R | GTTTGCCGTTCTGCATGATAG | ||
Dicer-2 | CDS_25848_transcript_29057 | qTt_Dcr2_F | GTTTGACTCAGGGAACGAAGA |
qTt_Dcr2_R | GAAGTGAGTTGACGACAGAGAG | ||
drosha | CDS_15925_transcript_14592 | qTt_drosha_F | TCTGCTTCTCGCTACGTTATG |
qTt_drosha_R | GTTTGCCGTTCTGCATGATAG | ||
Ago-1 | CDS_15926_transcript_14593 | qTt_Ago1_F | CTCTCCCGAATTCACGACTAAC |
qTt_Ago1_R | ACTGTCTGCCTTGATCCAATAC | ||
Ago-2 | CDS_6124_transcript_5961 | qTt_Ago2_F | TGGGAGAGGTTGATCCTTGTA |
qTt_Ago2_R | ACAGGTTTCTGTCGGAATATGG | ||
Alg | CDS_16858_transcript_15514 | qTt_Alg_F | CATCATTCCATTCCCGCTGATA |
qTt_Alg_R | CCAGAAGCATCCACTCTTGTT | ||
Piwi | CDS_26052_transcript_29621 | qTt_Piwi_F | CATCATTCCATTCCCGCTGATA |
qTt_Piwi_R | CCAGAAGCATCCACTCTTGTT | ||
Aub | CDS_18257_transcript_16934 | qTt_Aub_F | GTTCCCGACATGAACAAGAAAG |
qTt_Aub_R | AGCTCTGGCGGAAAGTATAAG | ||
SpindleE | CDS_828_transcript_1424 | qTt_SpindleE_F | TCGCAAGGCACTCTCTACTA |
qTt_SpindleE_R | CAGAGGACAGCGTTGATTGT | ||
Tdr | CDS_9987_transcript_9161 | qTt_Tdr_F | GTCATCACACCAGATCCTAACC |
qTt_Tdr_R | GGATACTGCGAGTTACCTCTTC | ||
Mut-7 | CDS_3222_transcript_3490 | qTt_Mut-7_F | TTATCTGTGCCGGTGGAATAC |
qTt_Mut-7_R | CCAAGCTACGATACCCTCTTG |