MEF2A alters the proliferation, inflammation-related gene expression profiles and its silencing induces cellular senescence in human coronary endothelial cells

Table 5 The primer sequences for real time quantitative PCR

Serial no.	Genes	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Tm (°C)
1	MEF2A	AGCAGCCCTCAGCTCTCTTG	GGTGAAATCGGTTCGGACTTG	60
2	PIK3CG	TCCTCTTTGTGATGGGAACTT	TGTGTGATGACGAAGGGCT	60
3	SERPIND1	TGATTCTCAACTGCATCTACTTC	CCTCTCTCTCATTCAGCCG	60
4	IL1B	ACAGTGGCAATGAGGATG	TGTAGTGGTGGTCGGAGA	60
5	ENPP1	ACTGCGAAAGTATGCTGA	TTCTTGGTTACGGATGAC	60
6	ZAK	GCGGTGGAGGAAGATTTG	TGACTGAGGACCCTGAGTATTT	60
7	FOS	TACTACCACTCACCCGAAGA	GAATGAAGTTGGCACTGGA	58
8	MMP1	AAACCCCAAAAGCGTGTG	GGGTAGAAGGGATTTGTGC	60
9	DQB1	CTGGTGATGATGGAAATGAC	TTGCTCTGGGCAGATTCA	60
10	GUCY1A3	GGAGCCGAGTCTATCTTCA	TGTGCTGTGCCAATGTTC	60
11	MYL9	AACGCCTTTGCCTGCTTC	CATCTCGTCCACTTCCTCATC	60
12	GJA5	GGCAAGGAAATAGTAGGG	TTGGCAGTCTCATTAGGG	60
13	CSF2	CCTGTGCAACCCAGATTA	TGGCTACCAGCAGTCAAA	60
14	TRIM	GGGTGTCCACATAATTGTTGT	GCCCTAACTGGTATGTTACG	60
15	MRPL45	GAAATGTTGTCAGGGTGTTG	CCTTAAAGTCACATCGCTCA	60
16	SGK196	ACCTGGAAGAAACACTAAACC	GGTCGTTGGAGTCGCACA	60
17	BCL2A1	CATCATTAACTGGGGAAGAA	ATTATGAACTCCGCAACAAA	60
18	NR5A2	CGTGGAGGAAGGAATAAG	TCAGGTCAGAGGGCATAG	60
19	ANK3	CCTATTTATGTTGATTGTTATGG	CTTGGCTGGTGTCTCTAATCT	60
20	PYHIN1	AAGGAGAATGCCACAATATC	TGTCTCTGGATGAAACTATGC	60

ISSN: 1471-2199