Table 1 Candidate reference genes, primer sequences, and characteristics of PCR amplification in Isatis indigotica
Gene symbol | Gene ID | Gene name | Primer: forward/reverse | Arabidopsis ortholog no. | Identify (%) | Amplicon size (bp) | E (%) | R 2 |
|---|---|---|---|---|---|---|---|---|
ACT | GARR01013235.1 | Actin | GCTCACGGAAGCACCT CGACCACTAGCGTAAAGT | At3g53750 | 95 | 128 | 91 | 0.9984 |
UBC | GARR01010405.1 | Ubiquitin-conjugating enzyme | TTTGCTGGAAAGGGAC TTGGAGGTTTGAAAGGAT | At1g50490 | 92 | 105 | 107 | 0.9944 |
α-TUB | GARR01008456.1 | Alpha-tubulin | GAGCCTTTGTTCATTG CAACCTCCTCATAATCC | At1g50010 | 83 | 102 | 109 | 0.9968 |
β- TUB | GARR01020827.1 | Beta-tubulin | TAAAGAAGTGGACGAAC CCCTTAGGAGGAATGT | At5g12250 | 88 | 111 | 96 | 0.9987 |
EF1-α | GARR01019351.1 | Elongation factor 1-α | GCCGATTGTGCTGTCC GTGGCATCCATCTTGTTA | At1g07930 | 96 | 149 | 94 | 0.9859 |
MUB | GARR01001157.1 | Membrane-anchored ubiquitin-fold protein | TTTCCCGATGCTACAA ACCTGAGGCTGAATGA | At5g15460 | 90 | 215 | 109 | 0.9969 |
CYP | GARR01006610.1 | Cyclophilin | GGCAAGACAGTTCCTA TGATTCTCCACCCATA | At2g29960 | 89 | 177 | 99 | 0.9975 |
RPL | GARR01002832.1 | Ribosomal protein L18 | CAAGGGCTAGGATTGA TAAGGTTTGGAGTGGC | At5g27850 | 90 | 172 | 106 | 0.9976 |
TIP41 | GARR01011419.1 | TIP41-like family protein | AACACTTGAGCAGCAA GTATGGCGTCGTAAAA | At4g34270 | 93 | 173 | 105 | 0.9980 |