Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune

Table 2 Expression stability of the nine candidate reference genes as calculated by NormFinder

Rank	ABA treatment		MeJA treatment		N treatment		Different tissues		All samples
Rank	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability	Gene	Stability
1	ACT	0.116	MUB	0.144	TIP41	0.049	EF1-α	0.041	MUB	0.188
2	EF1-α	0.184	α-TUB	0.153	EF1-α	0.075	CYP	0.041	EF1-α	0.191
3	MUB	0.247	EF1-α	0.271	CYP	0.098	α-TUB	0.084	ACT	0.327
4	UBC	0.275	TIP41	0.382	MUB	0.123	ACT	0.127	CYP	0.366
5	CYP	0.510	β-TUB	0.409	ACT	0.128	MUB	0.134	α-TUB	0.433
6	RPL	0.551	RPL	0.511	β-TUB	0.279	RPL	0.232	RPL	0.440
7	α-TUB	0.592	CYP	0.574	UBC	0.322	β-TUB	0.662	β-TUB	0.541
8	TIP41	0.633	ACT	0.614	RPL	0.364	UBC	0.753	TIP41	0.694
9	β-TUB	0.782	UBC	1.683	α-TUB	0.464	TIP41	1.172	UBC	0.942

ISSN: 1471-2199