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Table 3 Expression stability of the nine candidate reference genes, as calculated by BestKeeper

From: Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune

Rank

ABA treatment

MeJA treatment

N treatment

Different tissues

All samples

Gene

CV ± SD

Gene

CV ± SD

Gene

CV ± SD

Gene

CV ± SD

Gene

CV ± SD

1

MUB

1.31 ± 0.29

TIP41

1.05 ± 0.25

CYP

0.29 ± 0.05

ACT

1.62 ± 0.34

MUB

3.21 ± 0.72

2

TIP41

1.45 ± 0.34

MUB

1.49 ± 0.35

TIP41

0.29 ± 0.06

MUB

1.66 ± 0.35

ACT

3.52 ± 0.80

3

ACT

1.72 ± 0.40

EF1-α

1.56 ± 0.30

MUB

0.32 ± 0.07

CYP

2.96 ± 0.60

TIP41

3.78 ± 0.85

4

CYP

2.42 ± 0.53

α-TUB

1.99 ± 0.44

EF1-α

0.47 ± 0.08

α-TUB

3.15 ± 0.63

α-TUB

4.99 ± 1.08

5

EF1-α

2.50 ± 0.47

ACT

2.30 ± 0.53

ACT

0.94 ± 0.21

TIP41

3.24 ± 0.69

UBC

4.96 ± 1.18

6

UBC

3.22 ± 0.78

CYP

2.45 ± 0.55

β-TUB

0.97 ± 0.24

EF1-α

3.67 ± 0.60

CYP

5.29 ± 1.12

7

α-TUB

4.20 ± .94

RPL

2.85 ± 0.62

UBC

1.18 ± 0.27

RPL

4.19 ± 0.78

EF1-α

5.69 ± 1.03

8

RPL

4.30 ± 0.89

β-TUB

2.88 ± 0.75

RPL

1.85 ± 0.41

β-TUB

4.32 ± 0.95

β-TUB

5.50 ± 1.35

9

β-TUB

4.40 ± 1.11

UBC

7.33 ± 1.83

α-TUB

2.08 ± 0.47

UBC

5.27 ± 1.22

RPL

6.38 ± 1.32