Strain or plasmid | Description | Reference or source |
---|---|---|
Strains | ||
 E. coli | ||
  DH5α | Host strain used for general subcloning | [61] |
  MC1061 | Host strain used for subcloning with Lc. lactis derived pNZ8048 vector; recA positive strain | Mobitec, Göttingen, Germany |
 Lactobacillus plantarum | ||
  423 | Probiotic with multiple adhesion genes and a plantaricin bacteriocin producer; forms part of the entiro™ probiotic; originally isolated from sorghum beer | Cipla Medpro (Pty.) Ltd. |
  423 pNZmazFnisRK | Contains the pNZmazFnisRK plasmid; CmR | This study |
 Enterococcus mundtii | ||
  ST4SA | Probiotic with multiple adhesion genes and a mundticin bacteriocin producer; forms part of the entiro™ probiotic; originally isolated from soybeans | Cipla Medpro (Pty.) Ltd. |
  ST4SA pNZmazFnisRK | Contains the pNZmazFnisRK plasmid; CmR | This study |
 Lactococcus lactis | ||
  pNZ9000 | Standard host strain for nisin regulated gene expression; harbors the nisR and nisK nisin regulatory genes integrated into the pepN gene locus | Mobitec, Göttingen, Germany |
  pNZ9000 pNZmazF | Contains the pNZmazF plasmid; CmR | This study |
Plasmids | ||
 pNZ8048 | Broad-host range vector; E. coli shuttle vector; LAB expression vector containing nisin A inducible-promoter (PnisA); CmR | Mobitec, Göttingen, Germany; [32] |
 pGKV223D | E. coli/LAB shuttle vector; LAB expression vector with L23 promoter; EmR | University of Gröningen, The Netherlands |
 pNZnisRK | pNZ8048 vector carrying the nisR and nisK regulatory genes for cloning in LAB strains that do not have the regulatory genes integrated onto the chromosome; CmR | This study |
 pNZmazF | pNZ8048 vector carrying the E. coli mazF toxin gene under the control of the PnisA promoter; CmR | This study |
 pNZmazFnisRK | pNZnisRK vector carrying the E. coli mazF toxin gene under the control of the PnisA promoter; CmR | This study |
 pNZmazFnisRKerm | pNZ8048nisRK vector carrying the E. coli mazF toxin gene under the control of the PnisA promoter and the erm gene; CmR, EmR | This study |
 pBluescriptKS | PCR cloning vector; AmpR | Stratagene, California, USA. |
 pKSFRT | pBluescriptKS plasmid carrying two flippase (FLP) recombination target sequences (FRT) in a direct repeat orientation; AmpR | This study |
 pKSFRTErm | pBluesriptKS plasmid carrying the erm gene flanked by two FRT sequences in a direct repeat orientation; EmR, AmpR | This study |
 pGKVPldhFLP | pGKV223D vector carrying the flippase (FLP) gene downstream of the constitutive L. plantarum 423 lactate dehydrogenase gene Pldh promoter; EmR | This study |
 pNZasRNA_repAnisRK | pNZmazFnisRK vector carrying a 350 bp asRNA_repA transcript downstream of the nisin-inducible PnisA promoter; CmR | This study |
 pNZasFLPasRNA_repA | pNZasRNA_repAnisRK vector carrying the flippase (FLP) gene downstream of the constitutive L. plantarum 423 lactate dehydrogenase gene Pldh promoter; CmR | This study |