Fig. 4

mRNA expression of MITF, TYR, TYRP1, and TYRP2 in alpaca melanocytes. a–d represent the expression of MITF, TYR, TYRP1, and TYRP2, respectively. The control group represents normal cultured melanocytes. The miRNA mimic group represents melanocytes transfected with miRNA mimic. The black column represents the alpaca melanocytes transfected with miR-101a-3p & miR-144a-3p (1:1) mimic. The light gray column represents the alpaca melanocytes transfected with miR-144a-3p mimic. The dark gray column represents the alpaca melanocytes transfected with miR-101a-3p mimic. The miRNA inhibitor group represents melanocytes transfected with miRNA inhibitor. The black column represents the alpaca melanocytes transfected with miR-101a-3p & miR-144a-3p (1:1) inhibitor. The light gray column represents the alpaca melanocytes transfected with miR-144a-3p and miR-101a-3p inhibitors (* means P < 0.05, ** means P < 0.01, *** means P < 0.001)