Fig. 7
qRT-PCR expression analysis of shoots and roots of wheat seedlings exposed to multiple heavy metals (Ni, Co and Cd). Five days old wheat seedlings were exposed to the mentioned heavy metals for the period of 14 days. Total RNA was extracted from the treated and control samples and 2 µg of RNA was used to construct the cDNA. Ct values were normalized against wheat ARF1 as an internal control. Fold expression values were calculated relative to the control tissue of the mentioned wheat ZIFL. Vertical bars represent the standard deviation. # represent the significantly difference at p < 0.05 with respect to their respective control treatments