Table 1 Oligonucleotide sequences used for Taqman PCR and siRNA sequences
From: The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
Primer name | Application | Sequence or assay ID |
|---|---|---|
1. Human BACE1-AS–F | Real-time PCR | GAAGGGTCTAAGTGCAGACATCTT |
2. Human BACE1-AS–R | Real-time PCR | AGGGAGGCGGTGAGAGT |
3. Human BACE1-AS–P | Real-time PCR | ACATTCTTCAGCAACAGCC |
4. Mouse BACE1-AS–F | Real-time PCR | GTAGGCAGGGAAGCTAGTACTGA |
5. Mouse BACE1-AS–R | Real-time PCR | AGAGGCTTGCAGTCCAGTTC |
6. Mouse BACE1-AS–P | Real-time PCR | CCTGGAAGGAGAAACAG |
7. Human BACE1–F | Real-time PCR | Taqman(Assay ID: Hs00201573_m1) |
8. Human BACE1–R | Real-time PCR | Taqman(Assay ID: Hs00201573_m1) |
9. Human BACE1–P | Real-time PCR | Taqman(Assay ID: Hs00201573_m1) |
10. Mouse BACE1–F | Real-time PCR | Taqman(Assay ID: Mm00478664_m1) |
11. Mouse BACE1–R | Real-time PCR | Taqman(Assay ID: Mm00478664_m1) |
12. Mouse BACE1–P | Real-time PCR | Taqman(Assay ID: Mm00478664_m1) |
13. Human BACE1-AS siRNA_a | siRNA | CCCTCTGACACTGTACCATCTCTTT |
14. Human BACE1-AS siRNA_b | siRNA | AGAAGGGTCTAAGTGCAGACATCTG |
15. Human BACE1-AS siRNA_c | siRNA | CCAGAAGAGAAAGGGCACT |