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Fig. 4 | BMC Molecular Biology

Fig. 4

From: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

Fig. 4

PSMD1 and PSMD2 overexpression regulates the number and size of cellular lipid droplets. a Detection of the cellular LDs marked by BODIPY493/503 through a laser scanning confocal microscope. The HepG2 cells were seeded on the slide in a 24-well plate. Then, the cells were transfected with PSMD1/PSMD2 or NC overexpression plasmid for 48 h. Subsequently, the cells were treated with 200 μM oleic acid for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI for observation by microscope. b, c The number of cellular LDs from different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 20; *p < 0.05; **p < 0.01) vs. NC. d, e The size of cellular LDs of different groups of cells in the absence or present of OA. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 10; *p < 0.05; **p < 0.01) vs. NC

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